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. 2016 Mar 21;27(11):3356–3367. doi: 10.1681/ASN.2015020177

Figure 7.

Figure 7.

HIF1 dependent and independent effects on glycolytic enzyme protein expression and phosphorylation of pyruvate dehydrogenase. (A) Western blotting for HIF1α, PFKP, PFKFB3, PKM2, and PKLR in two groups of BUMPT cultured proximal tubule cells without and with HIF1α shRNA knockdown exposed to 0.5% O2 for 48 hours. (B) Western blotting for HIF1α, phosphopyruvate dehydrogenase E1α (pPDHE1α; S293), and PDHE1α in two groups of BUMPT cultured proximal tubule cells without and with HIF1α shRNA knockdown exposed to 0.5% O2 for 48 hours. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.