Figure 2.

DCA prevents CP-induced renal morphologic changes. Representative photomicrographs (magnification ×630) of hematoxylin and eosin staining in paraffin-embedded kidney sections 72 hours after CP administration from mice treated with (A) saline, (B) DCA, (C) CP, or (D) CP and DCA, as outlined in the CP-induced AKI model section in the Concise Methods. Circles indicate glomeruli. Arrows indicate condensed nuclei of apoptotic proximal tubule cells. Insets in (C) show detail of apoptotic nuclei. Bar=50 µm. (E) ATN scoring of histopathologic features in kidney sections. The scoring system is outlined in the Concise Methods. Results are expressed as mean±SEM, n=6 animals per group. ***P<0.001.