Figure 3.

DCA decreases renal cell apoptosis in CP-induced AKI. Representative photomicrographs (magnification ×100) of TUNEL staining in the kidneys of mice treated with (A) sal, (B) DCA, (C) CP, and (D) CP and DCA, as outlined in the CP-induced AKI model section in the Concise Methods, 72 hours after CP administration. Green fluorescent staining indicates TUNEL-positive nuclei. Bar=100 µm. (E) Quantitative analysis of TUNEL-positive cells. Results are expressed as mean±SEM, n=8 animals per group. ***P<0.001.