Figure 4.

DCA attenuates CP-induced p53-mediated apoptotic signaling. Mice were treated with sal, DCA, CP, or CP and DCA, as outlined in the CP-induced AKI model section in the Concise Methods. (A) Renal caspase 3 activity expressed as mean±SEM, n=6 animals per group. (B) Transcript levels of trp53 in the kidneys, derived from transcriptome data, expressed as trp53 transcripts per million. Results are expressed as mean±SEM, n=3 animals per group. (C) Representative immunoblot of kidney lysates for phospho-p53 (Ser15) and total p53 with β‑actin indicating equal protein loading. ***P<0.001; **P<0.01. RFU, random fluorescence units.