Figure 3.
Kmo-KO mice develop albuminuria and foot process effacement. Female (A) and male (B) Kmo-KO mice (C57BL/6N-Kmotm1a(KOMP)Wtsi) were examined for development of albuminuria (black bars). Both groups develop albuminuria as early as 4 weeks after birth compared with wildtype animals (gray bars). Albuminuria does not change significantly between 4 and 10 weeks. Morphologic analysis using light microscopy revealed no obvious differences between wildtype (C) and Kmo-KO animals (D) at 10 weeks of age (periodic acid–Schiff staining; size bar represents 50 μm). TEM analysis showed only a partial podocyte foot process effacement in some areas in the KO mice (panels F/F′), whereas the majority of examined glomeruli displayed normal foot processes comparable to the wildtype animals (panels E/E′).