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. 2016 Jun 20;12(10):2512–2518. doi: 10.1080/21645515.2016.1191719

Figure 4.

Figure 4.

Cimetidine reduces Foxp3 by modulating the PI3K-Akt pathway. A. Decrease in Foxp3 is accompanied by increase in pAkt during stimulation with CIM. HA-Foxp3 Jurkat T cells were stimulated with CIM (5 μg/ml) for 0, 4, 8 or 12 h then cell lysates were normalized for protein content and subjected to Western Blotting of the Foxp3 (HA), pAkt, Akt and β-actin (left). The expression of Akt and pAkt relative to β-actin was analyzed as intensity ratio by Image-Pro Plus (Right). B. Inhibition of PI3K prevents the decrease of Foxp3 that is induced by stimulation with CIM. HA-Foxp3 Jurkat T cells were stimulated with CIM (5 μg/ml) for the indicated periods in the presence or absence of PI3K blocker LY294002 (50 nM). Cell lysates were normalized for protein content and subjected to Western Blotting. Image-Pro Plus was employed to analyze the relative ratio for HA/β-actin (C) and Stub1/β-actin (D). Data are expressed as means with SD from 3 independent experiments. Statistical significance was calculated using t-test (**P< 0.01 and *P < 0.05).