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. 2016 Oct 28;11(10):e0165464. doi: 10.1371/journal.pone.0165464

Fig 4. Expression analysis of 3D-cultured NCCs compared to non 3D-cultured NCCs and cadaveric human corneal keratocytes.

Fig 4

(A) qPCR analysis showed 3D cultured derived NCCs had lower levels of expression of many neural crest markers, such as PAX3, SOX10, CX32, FOXD3 and MSX1, compared to non-3D cultured NCCs. Genes that are highly expressed in corneal keratocytes were up-regulated by 3D culture. (B) The gene expression profile of 3D-cultured NCCs was largely incompatible with cadaveric corneal keratocytes. Most genes assayed had significantly higher or lower levels of gene expression when the two populations were compared.