Fig 3. Normal integrin activation and degranulation in isolated Pdlim7^-/- platelets.

WT (dark grey bar) and Pdlim7^-/- (light grey bar) washed platelets were stimulated with the agonists thrombin (A, C) and CRP (B, D). Flow cytometric analysis was performed to determine integrin activation (A, B; binding of JON/A-PE) and degranulation (C, D; surface exposure of P-selectin) in response to various agonist concentrations. Results are given as mean fluorescence intensities (MFI) ± SD of 5 mice per genotype and are representative of n = 5 (JON/A-PE) and n = 6 (anti P-selectin-PE), respectively, individual experiments. Abbreviations: CRP: collagen related peptide (AYPGKF).