Table 1. Ability of individual primer combinations to generate polymorphic products that distinguish the original cultivar from the in vitro generated somaclone.
| Used isoschizomer sensitive to DNA methylation / primer combination* | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Msp/ I | Msp/II | Msp/III | Msp/IV | Msp/V | Msp/VI | Msp/VII | Msp/VIII | Msp/IX | Hpaa/I | Hpaa/II | Hpaa/III | Hpaa/IV | Hpaa/V | Hpa/VI | Hpa/VII | Hpa/VIII | Hpaa/IX | |
| Number of polymorphic amplicons (within one cultivar) | 1+0b | 0+1 | 1+0 | 0+1 | 2+0 | 1+0 | 0 | 1+1 | 0 | 11 +2 | 1+5 | 7+2 | 3+6 | 2+3 | 0+3 | 3+0 | 3+0 | 1+3 |
| Number of polymorphic amplicons (within both cultivars) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1+1 | 1+0 | 0 | 1+1 | 0 | 0 | 0 | 0 | 0 |
| Total | 1 | 1 | 1 | 1 | 2 | 1 | 0 | 2 | 0 | 15 | 7 | 9 | 11 | 5 | 3 | 3 | 3 | 4 |
* Sequences of individual primer combinations are specified in the Material and Methods section.
a—Samples ultimately selected for use in subsequent deep amplicon sequencing.
b–For each summation in the table, the first number represents the number of amplicons newly appearing in somaclones, and the second number represents the number of amplicon that disappeared in the somaclones when compared with the standard cultivar.