Table 2. The primers linked to resistance genes Sr25, Sr26, Sr28, Sr31, Sr32 and Sr38.
Tagged Sr genes | Marker | Size of markers (bp) | Primer sequence | References |
---|---|---|---|---|
Sr25 | Xwmc221 | 190 |
F-5′ACGATAATGCAGCGGGGAAT
R-5′ GCTGGGATCAAGGGATCAAT |
21 |
Sr26 | Sr26#43 | 207 |
F-5′AATCGTCCACATTGGCTTCT
R-5′ CGCAACAAAATCATGCACTA |
24 |
Sr28 | Wpt-7004 | 194 |
F-5′CTCCCACCAAAACAGCCTAC
R-5′ AGATGCGAATGGGCAGTTAG |
25 |
Sr31 | SCSS30.2576 | 576 |
F-5′GTCCGACAATACGAACGATT
R-5′ CCGACAATACGAACGCCTTG |
26 |
Sr32 | csSr32#1 | 184 |
F-5’GGTTTGGTGGCAACTCAGGT
R-5’ CATAAGCCAAAGAGGCACCA |
27 |
Sr38 | Xcmwg682 | 259 |
F-5’AGGGGCTACTGACCAAGGCT
R-5’TGCAGCTACAGCAGTATGTACACAAAA |
29 |