Skip to main content
. 2016 May 9;7(23):33512–33528. doi: 10.18632/oncotarget.9257

Figure 2. L-asparaginase (Asp) treatment reduces the mitochondrial membrane potential and expression of anti-apoptotic Bcl-2 family proteins.

Figure 2

A., Representative flow plots of SF188 glioblastoma cells treated with increasing concentrations of L-asparaginase prior to staining for JC-1 and flow cytometric analysis. Treatment with the mitochondrial uncoupler carbonyl cyanide 3-chlorophenylhydrazone (CCCP) served as positive control. B.-E., T98G (B), U251 (C), LN229 (D) and SF188 (E) glioblastoma cells were treated as indicated with L-asparaginase under serum starvation (1.5% FBS). Whole-cell extracts were examined by Western blot for Mcl-1, Bcl-2, Bcl-xL, Noxa and Usp9X. Actin served as a loading control. F., T98G glioblastoma cells were treated for 6 or 24h with increasing concentrations of L-asparaginase prior to performing rtPCR for Mcl-1 and Noxa. Columns, mean. Bars, SD.