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. 2016 May 26;7(23):33722–33743. doi: 10.18632/oncotarget.9591

Figure 6. Schematic representation of in vivo studies.

Figure 6

A. E4 pretreatment (neuroprotective effect). Newborn rat pups at P4 were assigned to: sham group (neither vehicle nor E4/steroids were applied), vehicle group or groups of animals pretreated either by 5 mg/kg/d E4 and 10mg/kg/d E4 alone or in combination either with 1.6mg/kg/day P4 and/or 136ng/kg/day E2 or with 16mg/kg/day P4 and/or 136ng/kg/day E2. From P4 to P7 inclusive, the rat pups were administered ip either vehicle or E4 alone or E4 combined with other steroids or neither by vehicle nor by other compounds (sham group). At P7, animals from the vehicle and steroid groups were subjected to hypoxic-ischemic (HI) insult. The sham group passed through anesthesia and skin incision but without HI insult or injections. Rat pups were sacrificed at P14. B. E4 treatment (therapeutic effect). Newborn rat pups at P7 were assigned to: sham group (neither vehicle nor E4/steroids were applied), vehicle treated group or groups of animals pretreated either by 5 mg/kg/d E4 and 10mg/kg/d E4 alone or in combination either with 1.6mg/kg/day P4 and/or 136ng/kg/day E2 or with 16mg/kg/day P4 and/or 136ng/kg/day E2. At P7, animals from the vehicle and steroid groups passed through hypoxic-ischemic (HI) procedures. Upon retrieval from hypoxia chamber rat pups were administered ip either vehicle or one of combinations of E4, P4 and/or E2. The sham group went through anesthesia and skin incision but without HI insult or injections. Rat pups were sacrificed a tP14.