Figure 1. Deficiency of both DGKα and ζ impaired CD8 T cell responses.

a.-d. CD45.1⁺CD45.2⁺ congenic mice iv injected with 1 × 10⁴ CD45.2⁺Vα2⁺CD8⁺ WT or DKO naïve OT1 T cells were infected with LM-OVA on day 0 and examined on indicated days later. a. Representative dot plots of RBC-depleted peripheral blood leukocytes (PBLs). Top panels: CD8 and TCRVα2 staining of PBLs. Bottom panels: Donor-derived CD45.1⁻CD45.2⁺ OT1 cells from the gated TCRVα2⁺CD8⁺ population. b. Percentages of OT1 T cells in PBLs. Bars represent mean ± SEM. c. Representative dot plots of splenocytes. d. Percentages of donor-derived OT1 T cells in splenocytes. e.-f. Thy1.1⁺Thy1.2⁺ congenic mice iv injected with a mixture of 5 × 10³ Thy1.1⁺ WT and 5 × 10³ Thy1.2⁺ DKO naïve OT1 T cells were infected with LM-OVA and analyzed similarly to the method described in a.-d. e. Representative dot plots of Thy1.1 and Thy1.2 staining in gated TCRVα2⁺ PBLs and splenocytes. f. WT to DKO OT1 ratios in blood and spleen from individual mice. Data shown are representative of two independent experiments. Each circle represents one recipient mouse injected with WT and/or DKO OT1 T cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Student's t test).