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. 2016 Mar 17;7(23):33744–33764. doi: 10.18632/oncotarget.8164

Figure 3. Differential effects of DGKαζ deficiency on OT1 T cell expansion in vitro and in vivo.

Figure 3

a.-e. Impaired expansion of DKO OT1 T cells following LM-OVA infection. a.-b. Frequencies of 7-AAD+ and Annexin V+ donor-derived OT1 T cells in PBLs on day 7 after LM-OVA infection. c.-d. Percentages of Ki67+ donor-derived OT1 T cells in PBLs on 6 day after infection (N = 7; **, P < 0.01 determined by Student's t test). e. In vivo assessment of donor-derived OT1 T cell proliferation. CFSE-labeled WT and DKO naïve OT1 T cells were adoptively transferred into recipient mice, followed by LM-OVA infection. Overlaid histograms show CFSE intensity in donor-derived OT1 T cells in splenocytes 72 hours after LM-OVA infection. Data shown are representative of two experiments. f. Enhanced TCR-induced DKO OT1 T cell proliferation in vitro. WT and DKO OT1 T cells from the spleen were CFSE labeled and stimulated with either anti-CD3 (0.1 μg/ml) or SIINFEKL peptide (1 ng/ml) in vitro for 48 and 72 hours. Overlaid histograms show CFSE intensity in gated WT and DKO OT1 T cells. Data are representative of two experiments.