Figure 6. Effects of DGKα and ζ double deficiency on memory CD8 T cell responses.

WT and DKO memory OT1 T cells sorted from splenocytes of recipients on day 35 after LM-OVA infection were transferred into secondary recipients (1 × 10⁴/mouse), which were subsequently infected by LM-OVA 18 hours after transfer. a. Representative dot plots and percentages b. of WT and DKO donor-derived CD45.1⁻CD45.2⁺ memory OT1 cells in PBLs and splenocytes in secondary recipients 7 days postinfection. c.-d. Splenocytes from secondary recipients were stimulated with SIINFEKL peptide in the presence of GolgiPlug for 5 hours, followed by cell surface and intracellular staining and FACS analysis. Representative contour plots c. and percentages of IFNγ⁺ or TNFα⁺ cells d. of donor-derived WT and DKO memory OT1 T cells are shown. Data shown represent two independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Student's t test).