Figure 7. Absence of IL-10 in DCs restored the capacity of cisplatin/LPS-primed DCs to stimulate Th1-type T cell proliferation.

OVA-specific CD4⁺ and CD8⁺ T cells were isolated; stained with CFSE; co-cultured for 96 h with WT and IL-10^−/− DCs treated with LPS (100 ng/ml), cisplatin (5 μg/mL), or LPS and cisplatin; and then pulsed with OVA_323-339 (1 μg/ml) for OVA-specific CD4⁺ T cells or OVA_257-264 (1 μg/ml) for OVA-specific CD8⁺ T cells. A. The proliferation of CD4⁺ and CD8⁺ T cells was then assessed by flow cytometry. B. The bar graphs represent the T cell types, such as T-bet⁺CD4⁺, GATA-3⁺CD4⁺ and RoRγt⁺CD4⁺ cells, that were present among the CFSE-labeled CD4⁺ T cells. C. The bar graphs show the percentages of Treg cells and IL-10-producing Tr1 cells among the CD4⁺ T cells. All bar graphs show the mean ± SD (n = 4 samples) from one representative plot out of two independent experiments. **p < 0.01 and ***p < 0.001 when comparing WT DCs and IL-10^−/− DCs after co-treatment with LPS and cisplatin. Treatments with no significant effect are indicated as n.s.