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(A) Images of H.E. and immunocytochemical staining for CK7, p63, ΔNp63, GATA-3, JAM-A and β-catenin in primary cultured cancer cells derived from HNSCC tissue. Bar: 100 μm. Western blotting (B) for p63, ΔNp63, GATA-3, JAM-A, β-catenin, occludin and claudin-1, 4, 7 and flow cytometry (C) for JAM-A HNSCC in primary cultured cancer cells transfected with siRNAs of p63, ΔNp63, GATA-3 and JAM-A.
