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. 2016 Mar 31;7(23):34011–34021. doi: 10.18632/oncotarget.8514

Figure 3. CDK4 is a direct target of miR-486-5p.

Figure 3

A. CDK4 WT 3′-UTR contains predicted miR-486-5p binding sites. The figure shows alignment of miR-486-5p with CDK4 WT 3′-UTR and the arrows indicate the mutagenesis nucleotides. B. Dual luciferase reporter assay. Luciferase reporter constructs containing pGL3-CDK4 WT 3′-UTR (CDK4 WT) and pGL3-CDK4 mut 3′-UTR (CDK4 mut) were co-transfected with miR-486-5p mimic or NC mimic in HEK293T cells. Relative firefly luciferase expression is displayed, normalized to Renilla luciferase expression. C. and D. The mRNA levels of CDK4 were measured by qRT-PCR and the protein levels were measured by western blot in H1299 and SPCA-1 cells transfected with NC and miR-486-5p mimic. *** P<0.001.