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. 2016 Mar 31;7(23):34011–34021. doi: 10.18632/oncotarget.8514

Figure 5. Knockdown of CDK4 can inhibit cell proliferation and impede cell-cycle progression in NSCLC cell lines.

Figure 5

A. and B. The mRNA levels of CDK4 were measured by qRT-PCR and the protein levels were measured by western blot and in H1299 and SPCA-1 cells transfected with negative control (siNC) and CDK siRNA (siCDK4). C. H1299 and SPCA-1 cells were transfected with siNC or siCDK4, and cell proliferation was determined by CCK-8. D. Colony formation assay in H1299 and SPCA-1 cells transfected with siNC or siCDK4. E. Cell cycle distributions of H1299 and SPCA-1 cells transfected with siNC or siCDK4 were detected by flow cytometry. * P<0.05, ** P<0.01, *** P<0.001.