Skip to main content
. 2016 Mar 31;7(23):34011–34021. doi: 10.18632/oncotarget.8514

Figure 6. The downregulation of miR-486-5p is due to the hyper-methylation of the miR-486-5p promoter region in NSCLC tissues and cell lines.

Figure 6

A. MiR-486-5p was upregulated in H1299 and SPCA-1 cells after 5 days of 5-AzaDc treatment. B. MS-PCR analysis of the miR-486-5p promoter in H1299 and SPCA-1 cells challenged with or without 5-AzaDc for five days. The methylation status of the miR-486-5p promoter region was detected using MS-PCR, where M indicates methylation and U indicates none. C. Primer sets used for amplification were designated as methylated (M) or unmethylated (U). Ten μL of PCR product was run on 1% agarose gel, stained with GoldView, and visualized under UV illumination. The numbers displayed indicate pairs of samples, with each pair comprising of one normal tissue (N) and one tumor tissue (T). ** P<0.01, *** P<0.001.