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. 2016 Apr 29;7(23):34371–34383. doi: 10.18632/oncotarget.9110

Figure 1. FRA1 is required for HNSCC cell proliferation.

Figure 1

(A) Cell growth analysis. FaDu cells were transduced in triplicates with lentivirus encoding shRNA targeting FRA1 (shFRA1) or nonsilencing control (shCon), or transfected with siRNA oligonucleotides targeting FRA1 (siFRA1) or non-silencing control (siCon). Graph represents average percentage of cell numbers normalized to that of control cells + SD at 72 h after seeding. Gene silencing was confirmed by immunoblotting as shown below each graph. (B) Cell cycle analysis by flow cytometry. Graph represents percentage of cells in G0/G1, S and G2/M phases + SD. (C) Immunoblotting for CyclinB1, CyclinD1 and Actin with protein lysates isolated at 48 h after gene transduction. (D) Soft-agar colony formation. Graph represents average number of colonies of triplicate dishes + SD. (E) Cell growth response to cisplatin. shCon or shFRA1 FaDu cells were treated in hexad with varying doses of cisplatin for 72 h, and then analyzed by MTT assay. Graph represents percentage of live cells normalized to that of untreated cells + SD. P-values were obtained via two tiered Student T-Test. Relative densitometry of immunoblots noted below each band was obtained by using Odyssey image tools.