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. 2016 Apr 23;7(23):34571–34581. doi: 10.18632/oncotarget.8952

Figure 4. ZBP-89, Bak and PDCD4 promoter activities were significantly increased after HIF-2α transfection in HepG2 and Hep3B cells.

Figure 4

HepG2 and Hep3B cells were transiently transfected with 0.4 μg of individual (ZBP-89 or Bak or PDCD4) promoter luciferase construct, pGL3 Basic or pGL3 control plasmids, together with 10 ng Renilla luciferase plasmid which served as an inner control for transfection efficiency and 0.8 μg pcDNA3.1 or pcDNA3.1- HIF-2α. The cells were lysed to determine the relative firefly luciferase activity, and the change of the activity was calculated in fold. The results were the mean of 3 independent experiments, and the values are expressed as mean ±SD (**P<0.01).