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. 2016 Apr 25;7(23):34759–34772. doi: 10.18632/oncotarget.8976

Figure 3. Putative binding proteins of USP19.

Figure 3

Immunoprecipitation and MALDI-TOF-MS analyses were performed to investigate novel substrates for USP19. A. USP19 overexpression or a control sample in 293T cells, which were immunoprecipitated with an anti-Myc antibody and stained with the silver staining method. B. and C. Bands showing differential expression were selected and analyzed by MALDI-TOF-MS analysis. D. The interaction between USP19 and CORO2A was confirmed by an immunoprecipitation assay with an anti-Myc antibody and immunoblotting with anti-Flag and anti-Myc antibodies using Myc-tagged USP19 and/or Flag-tagged CORO2A overexpressed 293T cell lysates. E. Reciprocal data for D were obtained with respective antibodies. F-H. 293T, 3T3-L1, and MCF7 cell lysates were precipitated by an anti-USP19 antibody. USP19 and CORO2A were detected by anti-USP19 and anti-CORO2A antibodies, respectively. F-H, 293T, 3T3-L1, and MCF7 cell lysates were precipitated by an anti-CORO2A antibody. CORO2A and USP19 were detected by indicated antibodies.