Figure 5. Genes essential for untangling chromatin and entrance into mitosis were also essential to prevent EDR.

A. Twelve of the validated genes have functions that untangle chromatin from G2 phase until metaphase, drive cells from G2 phase into mitotic prophase, and assemble the mitotic spindle during the prophase to prometaphase transition (illustrated in B. dark boxes, light font). The results of the three validation assays (TP53+ cells, TP53+ cells +ZVAD, and TP53- cells) carried out on each gene are summarized in panel A, and the FACS profiles are in Supplementary Figure S2B, S2C. FACS profiles for the validation assay with the strongest EDR signals are in panel C. The FACS profiles are consistent with induction of endoreplication or mitotic slippage when one of these genes is depleted.