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. 2016 Apr 28;7(23):35188–35198. doi: 10.18632/oncotarget.9072

Figure 4. AMPK is a direct target of miR-101 in breast cancer.

Figure 4

A. Predicted binding between mir-101-3p and the seeds matched in the 3′-UTRs of AMPK. B. Luciferase assay of MDA-MB-231 cells cotransfected with mir-101-3p mimics, and a luciferase reporter containing AMPK 3′-UTR (LDHA wt) or mutant constructs (AMPK mut). C. MDA-MB-231 and MDA-MB-468 cells were transfected with mir-101-3p mimics or scrambled oligonucleotide. mir-101-3p overexpression inhibited the AMPK mRNA expression. D. MDA-MB-231 and MDA-MB-468 cells were transfected with mir-101-3p mimics or scrambled oligonucleotide. mir-101-3p overexpression inhibited the protein expression of AMPK. β-actin was used as a loading control. All of the data are shown as the means ± s.e.m. ** P < 0.01.