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. 2016 Apr 29;7(23):35224–35232. doi: 10.18632/oncotarget.9109

Figure 5. Suppression of TCam-2 cell proliferation by TPH1 knockdown.

Figure 5

A. Confirmation of TPH1 knockdown efficiency by quantitative RT-PCR. TPH1 siRNA or control siRNA was transfected to TCam-2 cells for 3 days. The mRNA levels of TPH1 were normalized to GAPDH. n = 2, *, p < 0.01. B. Serotonin levels in TCam-2 cell culture supernatants were exarmined by ELISA. For TPH1 knockdown, siRNA was transfected to TCam-2 cells for 3 days. DHT was added to the cultured medium at a concentration of 10−7 M for 24 hrs. C. TCam-2 cells were transfected with control siRNA or TPH1 siRNA for 3 days. After that, the number of living cells was examined by measurement of absorbance (450 nm). Data are presented as mean ± s.d. (n=3), *, p < 0.01.