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. 2016 May 2;7(23):35257–35269. doi: 10.18632/oncotarget.9135

Figure 3. WSB1 is a bona fide target gene of miR-592 and functional downstream of miR-592 in HCC cells.

Figure 3

A. Sequence alignment of wild-type (WT) and mutated (Mut) putative miR-592-binding sites in the 3′-UTR of WSB1. B. Relative luciferase activities of plasmids carrying WT or mut WSB1 3′-UTR in SK-Hep-1 and SMMC7721 cells cotransfected with miR-592 mimics or NC. C. The expression levels of WSB1 in SK-Hep-1 and SMMC7721 cells were analyzed using western blot. D. SK-Hep-1 cells were co-transfected with miR-592 mimics or NC and WSB1 plasmid without 3′-UTR, the expression levels of WSB1 were analyzed using western blot. The effect of miR-592 and WSB1 on E. HCC cell proliferation and F. cell cycle progression. The mean and SD of three independent experiments performed in triplicate are shown.