Figure 1. IRX1 overexpression in HEK293T cells.

A. Transiently transfected HEK293T cells expressing either IRX1::GFP or GFP were FACS-sorted. Total RNA from 2 × 10⁷ GFP-positive cells was isolated and used for hybridization on Affymetrix HG-U133Plus 2.0 microarrays (n=3). More than 8,000 genes were differentially expressed. We shortened the list of genes for experimental work on those which were deregulated more than 4-fold (up: n=244; down: n=124). All genes of this highly deregulated group were annotated according to GO terms. Nearly a quarter of genes encoded for transcription factors. The number of up- and downregulated genes is indicated. B. The normalized Affymetrix chip hybridization signals (relative light units RLU) are displayed for MEIS genes, PBX genes and genes of the HOXA/B/C/D cluster. All genes displayed were significantly deregulated.