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. 2016 May 9;7(23):35341–35352. doi: 10.18632/oncotarget.9241

Figure 3. Chromatin-IP experiments to functionally investigate the IRX/MLL-AF4 interaction at MLL-AF4 target promotors.

Figure 3

Either mock-, IRX1-, MLL-AF4- or IRX1/MLL-AF4-transfected cell lines were investigated by ChIP. ChIP experiments were performed by using different antibodies (IgG, mCh (mCherry), MLL-C and IRX1). One representative experiment measured in triplicates is displayed. A. Experiments on the HOXA promotors without (left panels) or B. with 300 nM TSA (right panels). Binding of IRX1 became enhanced at these promotors when MLL-AF4 was present in the cell. Binding of MLL-AF4 to these promotors was very strong, independent of IRX1. Binding of wildtype MLL was enhanced in the presence of IRX1, but reduced in the presence of MLL-AF4. Upon TSA administration, binding of MLL-AF4 became reduced but retained in the presence of IRX1.