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. 2016 Jun 6;7(23):35497. doi: 10.18632/oncotarget.9855

Figure 5. Validation of anti-tumour effects of cetuximab and bevacizumab mediated through altered cellular metabolism.

Figure 5

Proteins were extracted from tumour xenografts for each treatment cohort (independent from the tumour xenograft lysates performed for proteomic profiling), obtained from pooled tumour xenograft samples from the validation experimental group (n=3). Immunoblotting analysis of the expression of ATP5B, SDC2, GPD2, ARF4 in both LIM1215 and HT-29 tumour xenograft lysates was performed (n=3; pooled for each treatment cohort, independent biological replicates performed for each antibody). Loading control (GAPDH; rabbit anti-GAPDH (Cell Signaling Technology; 1:1000)).