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. 2016 Nov 3;1(18):e88646. doi: 10.1172/jci.insight.88646

Figure 1. ATF4 haploinsufficiency attenuates medial calcification.

Figure 1

(A) Representative photographs (original magnification, ×10) of the lesions in aortic sinuses stained with von Kossa from 8-week-old Atf4+/+ and Atf4+/– mice (n = 8–10) on DBA/2J background. Mice were subjected to either sham operation for normal kidney condition (NKD) or 5/6 nx for chronic kidney disease condition (CKD). The mice were sacrificed 12 weeks after the surgeries. (B) Quantitative analysis of calcified lesions in the aortic sinus. More NKD data are shown in Supplemental Figure 1G. (C) Aortic calcium content in Atf4+/– mice. (D) Representative micrographs show more TUNEL-positive signal (pink) in nuclei (blue) of aortic sinus lesions from Atf4+/+ mice than from Atf4+/– mice. (E) Quantitative analysis of TUNEL-positive nuclei (pink) conducted on lesions from Atf4+/– mice. (F) Immunofluorescence analysis of CHOP (pink) in the aortic sinuses of Atf4+/– mice. One-way ANOVA with a Student-Newman post-hoc test was used for comparison between Atf4+/+ and Atf4+/– mice. Two-way ANOVA was used for comparison between NKD and CKD. **P < 0.01, ***P < 0.001. Scale bar: 100 μm.