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. 2016 Sep 26;17(10):1493. doi: 10.3390/ijms17101493

Figure 4.

Figure 4

EPHA7 is a direct target of miR-944 in NSCLC. (A) The expressions of EPHA7 were examined by qRT-PCR in NSCLC cell lines; (B) The expressions of EPHA7 were examined by western blot in NSCLC tissues and noncancerous lung tissues; (C) The relationships between miR-944 and EPHA7 protein levels were analyzed using Pearson’s method in NSCLC tissues, R = −0.48, p < 0.05; (D) The mRNA and protein levels of EPHA7 were measured in EPLC-32M1 and XLA-07 cells transfected with the miR-944 mimic and negative control (NC); (E) Sequences of the putative miR-944 binding sites; (F) The relative luciferase activity was determined using luciferase reporters with EPHA7-3′UTR in EPLC-32M1 and A549 cells transfected with the miR-944 mimic and negative control (NC); (G) The EPHA7 expression was analyzed using qRT-PCR and western blot in EPLC-32M1 and XLA-07 cells transfected with the siRNA (si-EPHA7) and negative control (NC); (H) The proliferation rates were analyzed using proliferation assay in EPLC-32M1 and XLA-07 cells after transfection with the siRNA (si-EPHA7) and negative control (NC). GAPDH was used as an internal control (B,D,G). The significance was evaluated by t-test (A,B,D,F–H), * p < 0.05.