Unweighted pair-group method with arithmetic (UPGMA) dendrogram of 28 Agaricus bisporus strains constructed by using genetic similarity analysis based on 17 new SSR markers. These strains are clustered into two groups: A (15 strains) and B (13 strains) at genetic similarity (GS) coefficient value 0.27. Group A are cultivated strains, including 1-CCMJ1009, 2-CCMJ1021, 3-CCMJ1020, 4-CCMJ1038, 5-CCMJ1033, 6-CCMJ1037, 7-CCMJ1039, 8-CCMJ1053, 9-CCMJ1013, 10-CCMJ1022, 11-CCMJ1028, 12-CCMJ1035, 13-CCMJ1343, 14-CCMJ1109, and 15-CCMJ1352. Group B are wild strains, including 16-CCMJ1106, 17-CCMJ1350, 18-CCMJ1361, 19-CCMJ1351, 20-CCMJ1377, 21-CCMJ1347, 22-CCMJ1360, 23-CCMJ1363, 24-CCMJ1369, 25-CCMJ1381, 26-CCMJ1384, 27-CCMJ1110, and 28-CCMJ1372.