Table 2.
Characteristics of the 17 polymorphic simple sequence repeats (SSR) markers of A. bisporus used in this study.
| Locus | SSR motif | Primer Sequence (5′–3′) | Ta (°C) | Na | Ne | Ho | He | PIC | I |
|---|---|---|---|---|---|---|---|---|---|
| AbSSR05 | (GATGAG)6 | F-CTCTGGGATATGGACGAGGA R-CCTCTTCACCTTGACCCTCA |
56 | 5 | 3.2197 | 0.5 | 0.702 | 0.635 | 1.3177 |
| AbSSR08 | (TGG)8 | F-GTAATGCTCCCGCTGTTGAT R-TCCGCTGTTCTTCCAACTCT |
56 | 4 | 2.3939 | 0.107 | 0.593 | 0.531 | 1.0785 |
| AbSSR10 | (CCA)8 | F-GAAGAATCACGGGTGAAGGA R-GAGGGCGATGTGACAGTTTT |
56 | 8 | 6.1455 | 0.423 | 0.854 | 0.816 | 1.905 |
| AbSSR14 | (TACC)6 | F-GGCAATCGGAAAGAACAAAA R-GCAGAGAACCATCCTCAACG |
56 | 7 | 4.2757 | 0.333 | 0.781 | 0.73 | 1.6081 |
| AbSSR15 | (TA)6 | F-GACTGCCTGATTGACGGATT R-TCCGACTCCGACATCCTATC |
56 | 6 | 4.2609 | 0.393 | 0.779 | 0.729 | 1.5613 |
| AbSSR17 | (CA)6 | F-GGACGAACTTATGCCGTGTT R-GGCACAGCCTGAGAGAGAAG |
56 | 2 | 1.6897 | 0 | 0.416 | 0.325 | 0.5983 |
| AbSSR18 | (GA)7 | F-CTCGAGTCGACGAAGGAAAC R-TCCTCGGTTTCGACTGTACC |
56 | 5 | 2.9418 | 0.786 | 0.672 | 0.611 | 1.2567 |
| AbSSR28 | (TC)12 | F-TGTCTGGTTTTGCTCACGTC R-TCAGCACACTTAATCGCACA |
56 | 4 | 2.5331 | 0.321 | 0.616 | 0.539 | 1.058 |
| AbSSR47 | (CA)8 | F-CATCGGAATCTGAGCTGTCA R-TGTGTCAAAAGTGGGTCCAA |
56 | 4 | 3.0519 | 0.308 | 0.686 | 0.607 | 1.1893 |
| AbSSR52 | (CAT)6 | F-TGGCTCTTTACAGCCTTGGT R-TGCAGATGTGGTAGGAGTTTTG |
56 | 6 | 3.3212 | 0.556 | 0.712 | 0.654 | 1.4004 |
| AbSSR75 | (CAA)7 | F-CGTCCAACATCAACGTCAAC R-GTGTACATCCCCTCGTCGTC |
56 | 6 | 5.0827 | 0.5 | 0.819 | 0.775 | 1.7012 |
| AbSSR85 | (CGT)5 | F-GACTGTTGACGTTTCGGGTT R-CAACGATGACCCGTTTTCTT |
56 | 3 | 2.1189 | 0 | 0.538 | 0.421 | 0.8165 |
| AbSSR87 | (CCT)6 | F-CAGTCGCACTCGAAATCGTA R-TTGTTGAGTGAGGCATCGAG |
56 | 5 | 1.6309 | 0.308 | 0.394 | 0.361 | 0.7984 |
| AbSSR89 | (CAT)7 | F-GATAGCTCCTGGTCACCGTC R-CTGGCTTCAAGAAGCGTACC |
56 | 6 | 4.0412 | 0.214 | 0.766 | 0.713 | 1.5111 |
| AbSSR111 | (GAG)12 | F-TGTCGATTGCGTCTTCTTTG R-CGCCTCGTTTCTCTACTTCG |
56 | 7 | 4.2151 | 0.536 | 0.777 | 0.728 | 1.6399 |
| AbSSR112 | (CAC)5 | F-TCACCCTCACTCAAACTCCC R-TCTCATCCGGTTCAACAACA |
56 | 3 | 2.8403 | 0 | 0.661 | 0.573 | 1.0695 |
| AbSSR159 | (GAA)6 | F-CGACCCATCATCAACTTCCT R-AACGAGGGAAAGGTCGATTT |
56 | 7 | 4.8246 | 0.143 | 0.807 | 0.763 | 1.697 |
| Mean | 5.1765 | 3.4463 | 0.3193 | 0.6807 | 0.6183 | 1.3063 | |||
Ta: annealing temperature; Na: observed number of alleles per locus; Ne: effective number of alleles per locus; Ho: observed heterozygosity; He: expected heterozygosity; PIC: Mean polymorphic information content; I: Shannon’s information index.