Figure 3.

The effect of SRSF2 silencing on the expression of genes involved in the regulation of apoptotic pathways in four renal cancer-derived cell lines. (A) Caki-2, UOK171, KIJ-265T, and KIJ-308T cells were transfected with SRSF2-specific (siSRSF2) or control (siControl) siRNA. The graphs show mean results of qPCR analysis of SRSF2 expression performed on cDNA from three independent biological experiments executed in triplicates; and (B) the expression of apoptotic genes in cells with silenced SRSF2 expression. Only genes that were positively validated in manual qPCR verification are shown. Statistical analysis was performed using t-test. The results for BCL2L2 in Caki-2 cells were analysed using Mann–Whitney test due to lack of normal distribution. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. NS: Lack of statistical significance.