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. 2016 Sep 22;17(10):1609. doi: 10.3390/ijms17101609

Figure 6.

Figure 6

Analysis of dRTM15 expression within transduced recessive dystrophic epidermolysis bullosa (RDEB) keratinocytes. (A) Schematic representation of dRTM15 including both binding domains (5′ and 3′) for trans-splicing induction. Arrows illustrate the binding positions of the primers used for RT-PCR. The dashed line represents the expected PCR product with a size of 506 nt; (B) gel electrophoresis showed full-length dRTM15 expression within transduced RDEB keratinocytes. A dRTM15 expression plasmid was additionally included as PCR template representing the positive control (dRTM15 pos.Ctrl.) at the expected size of 506 nt. cDNA of untransduced RDEB keratinocytes (RDEB) served as negative control. All PCR products were confirmed by Sanger sequence analysis.