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. 2016 Sep 23;17(10):1617. doi: 10.3390/ijms17101617

Figure 4.

Figure 4

Overexpression of lncCAMTA1 promotes HCC cell proliferation, CSC-like properties, and tumorigenesis. (A) lncCAMTA1 expression in HCCLM3 cells stably overexpressing lncCAMTA1 and control HCCLM3 cells; (B) Cell proliferation of HCCLM3 cells stably overexpressing lncCAMTA1 and control HCCLM3 cells was detected by cell counting kit-8 (CCK-8) assay, and the relative number of cells compared to day 0 is presented; (C) Cell proliferation of HCCLM3 cells stably overexpressing lncCAMTA1 and control HCCLM3 cells was detected by 5-ethynyl-2′-deoxyuridine (EdU) incorporation assay. Scale bars = 100 μm; (D) Spheroid formation assays showed that overexpression of lncCAMTA1 increased the number of spheroids generated from HCCLM3 cells; (E) Overexpression of lncCAMTA1 upregulated the expression of stem cell markers and transcription factors CD133, CD13, OCT4, SOX2, and NANOG. For A–E, data are shown as mean ± SD. ** p < 0.01 by Student’s t test, *** p < 0.001 by Student’s t test; (F,G) Overexpression of lncCAMTA1 promoted in vivo tumor growth of HCCLM3 cells. HCCLM3 cells stably overexpressing lncCAMTA1 and control HCCLM3 cells were subcutaneously injected into nude mice. Tumor volumes were measured every 7 days (F), and tumor weights were measured at 28 days after injection (G). n = 6 mice in each group, ** p < 0.01 by Mann–Whitney test. oeVec: empty vector control overexpression; oelncCAMTA1; lncCAMTA1 overexpression; OD: optical density.