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. 2016 Sep 27;17(10):1640. doi: 10.3390/ijms17101640

Scheme 1.

Scheme 1

Preparation of bovine NP cultures: Bovine tails were dissected and NP tissue was isolated using an 8-mm biopsy punch. NP tissue was placed in 30% w/v polyethylene glycol (PEG) solution (100 min) to reduce water content. NPs packed in regenerated cellulose membranes and Dyneema jackets were sutured and placed in culture media.