(A) Cytotoxicity of DEN (Dentatin) against human colon cancer cells (HT29). The cells were plated in 96-well plates and then exposed to 100, 50, 25, 6.25, 3.125 and 1.25 µg/mL of DEN for 72 h. The viability of treated cells were measured by using an MTT assay. Mean ± standard deviation (SD). (n = 3 well/treatment). * p < 0.05 compared with untreated cells; (B) Cytotoxicity of DEN-HPβCD (hydroxypropyl-β-cyclodextrin) complex against human colon cancer cells (HT29). The cells were plated in 96-well plates and then exposed to 100, 50, 25, 6.25, 3.125 and 1.25 µg/mL of DEN for 72 h. The viability of treated cells were measured by using an MTT assay. Mean ± standard deviation (SD). (n = 3 well/treatment). * p < 0.05 compared with untreated cells.