Figure 1.

Evaluation of DMD sgRNA:Cas9-mediated modifications of DMD in pig parthenogenetic embryos. (a) Schematic diagram of the pig DMD partial protein coding region and the DMD sgRNA:Cas9 targeting site; (b) Polymerase chain reaction (PCR) products of the DMD targeting region in pig embryos. MK, DNA marker; Con, PCR product of the targeted region amplified from wild-type pig; (c) Detection of sgRNA:Cas9-mediated on-target cleavage of DMD by T7EN1 cleavage assay in pig embryos; (d) Representative sequencing chromatographs of PCR product. Blue background indicates the DMD sgRNA sequence. Red triangle indicates cleavage site mediated by DMD sgRNA:Cas9. Green background indicates PAM construct.