Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Dual staining of L929 cells with Annexin V (AnV) and propidium iodide (PI). L929 cells were treated with 10 ng/mL TNF (T), 10 μM Z-VAD-fmk (V), 10 μg/mL cycloheximide (C), or 10 μM necrostatin-1 (N) for the indicated times. AnV+/PI−: early necroptosis; AnV+/PI+: necrosis (late necroptosis). The graph shows the average of three independent experiments for AnV+/PI+ cells. * p < 0.05 compared to T2h; + p < 0.05 compared to T3h; # p < 0.05 compared to 3 h without necrostatin-1.
