Figure 7.

Induction of apoptosis in TNF-treated L929 cells in the presence of necrostatin-1 (dual staining with Annexin V and propidium iodide). L929 cells were treated with 10 μM necrostatin-1 (N), 10 ng/mL TNF (T), 10 μg/mL cycloheximide (C) with or without 10 μM Z-VAD-fmk (V) or 100 μM Z-Asp-CH₂-DCB (A) for the indicated times. AnV⁺/PI⁻: early apoptosis or early necroptosis; AnV⁺/PI⁺: necrosis (late apoptosis or late necroptosis). The graph shows the average of three independent experiments for AnV⁺/PI⁺ cells. * p < 0.05 compared to NTC4h; ⁺ p < 0.05 compared to NTC5h.