Table 3.
Variabilities in the methods used for T cell receptor excision circles (TREC) estimation.
| TREC Methods | Research Groups |
|---|---|
| Detection method | |
| Semi quantitative PCR | Douek et al., 1998, Douek et al., 2000 |
| Hybridization probe | Jimenez et al., 2006, Loeffler et al., 2002 |
| Molecular beacon | Zhang et al., 1999 |
| TaqMan probe | Hazenberg et al., 2000, Przybylski et al., 2007, Mensen et al., 2013, Lorenzi et al., 2008, Sugita et al., 2008, Sairafi et al., 2012 |
| SYBR Green PCR | Ponchel et al., 2003 |
| PCR-ELIZA | Al-Harthi et al., 2000 |
| Cell population | |
| CD3 | Ringhoffer et al., 2013, Jimenez et al., 2006, Sairafi |
| CD4/CD8 | Douek et al., 1998, Fallen et al., 2003, Sugita et al., 2008 |
| Peripheral blood mononuclear cells | Przybylski et al., 2007, Douek et al., 2000, Eyrich et al., 2005 |
| Type of TREC | |
| sj TREC | The majority |
| sjTREC/βTREC | Dion et al., 2004, Ringhoffer et al., 2013, Ferrando-Martinez et al., 2010 |
| Result expression | |
| TREC/cell count | Przybylski et al., 2007 |
| TREC/mL or μL of Blood | Lorenzi et al., 2008, Eyrich et al., 2005, Chen et al., 2005 |
| TREC/μg DNA | Jimenez et al., 2006, Douek et al., 2000 |
| Absolute TREC count | The majority |
| Relative (Delta Ct method) | Uzunel et al., 2014, Sairafi et al., 2012 |