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. 2016 Oct 20;17(10):1752. doi: 10.3390/ijms17101752

Figure 2.

Figure 2

Screening of predicted miRNAs for targeting ASK1. (A) Candidate miRNAs that target ASK1 were selected based on prediction databases (www.TargetScan.org & www.microRNA.org); (B) ASK1 expression in ASCs transfected with the indicated candidate miRNAs was analyzed by immunoblot (# p < 0.05, ** p < 0.01, * p < 0.001 vs. Control, N.C.: negative control); (C) Luciferase assay using the 3’untranslated region (3′ UTR) of ASK1 was performed to confirm the interaction between miRNA-301a and ASK1 (# p < 0.05 vs. Control); (D) Endogenous expression of miRNA-301a was measured by real-time PCR (* p < 0.001 vs. Control).