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. 2016 Oct 21;17(10):1761. doi: 10.3390/ijms17101761

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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CERT gene disruption by the TALEN–CERT pair in the TAL-CERS2#18 clone. (A) The target site of the TALEN–CERT pair in the human CERT gene and indel analysis of the TAL-CERS2#18-CERT#10 (#18-10) clones. The target sequences (CERT Fw and Rv) are located in exon 2, which codes for part of the pleckstrin homology (PH) domain. Deletions and replacements are shown in red and their lengths are specified on the right of the sequences. WT, wild type; (B) Western blot analysis of CERT in the TAL-CERS2#18 clones. Cell lysates from the parent cells and the indicated mutants were immunoblotted with anti-CERT antibody (top) and anti-α-tubulin antibody (bottom). The arrow indicates CERT at 68 kD. P, parent cell line; 18, TAL-CERS2#18 clone; 18-10, TAL-CERS2#18-CERT#10 clone.