Figure 5.

The effects of phospholipase A₂ (PLA₂) inhibitors on TCTP-induced dopamine release from PC12 cells. (A,B) PC12 cells were pre-incubated for 20 min at 37 °C with 25 µM AACOCF₃, 10 µM TEA-PC, or 25 µM BEL, and then treated with 50 µg/mL MrTCTP for an additional 20 min in Ca²⁺-containing (+Ca²⁺), or Ca²⁺-free (−Ca²⁺) buffer. The amount of [³H]dopamine released into the culture supernatant was measured. * p < 0.05 relative to control release; # p < 0.05 relative to MrTCTP-treated release; (C) the fluorescence experiments were carried out at room temperature in the Ca²⁺ (2 mM CaCl₂)-containing buffer. PC12 cells were loaded with 5 µM Fura-2-AM for 30 min and then washed multiple times. The cells were pretreated with 25 µM BEL for 5 min, and then exposed to 50 µg/mL MrTCTP.