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A) Secondary structure of the E. coli GAC U1061A, with phylogenetically conserved bases in magenta and tertiary contacts observed in the cocrystals indicated by lines. Numbered nucleotides were substituted with 2-aminopurine. B) Sequences of hairpin constructs with sites of 2AP substitution in green. C) Cocrystal structure (1HC8) of E. coli GAC with 2AP substitution sites in green.
