. Author manuscript; available in PMC: 2017 Nov 6.

Published in final edited form as: J Mol Biol. 2016 Sep 29;428(22):4490–4502. doi: 10.1016/j.jmb.2016.09.015

Table 1.

Relaxation times for 2AP-GAC folding transitions.

	τ₁ (sec)		τ₂ (sec)		τ₃ (sec)
Construct	Global	1089AP	Global	1089AP	Global	1089AP
3 mM Mg²⁺	0.018	--	2.1	3.8	22	26
5 mM Mg²⁺	0.022	--	1.4	2.7	28	28
8 mM Mg²⁺	0.024	0.007	1.2	2.0	23	25
20 mM Mg²⁺	0.033	0.011	1.1	1.7	24	22
40 mM Mg²⁺	0.031	0.014	1.0	1.4	23	20
80 mM Mg²⁺	0.034	0.024	0.95	1.4	21	21
100 mM Mg²⁺	0.034	0.027	0.97	1.3	21	21

Relaxation times calculated from global fitting and independent A1089AP curve fitting. The error generated by Origin software from the curve fit is uniformly <5% of the parameter value. 20° C in 100 mM KCl, 10 mM sodium cacodylate, pH 6.5.