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. Author manuscript; available in PMC: 2017 Oct 28.
Published in final edited form as: Circ Res. 2016 Sep 22;119(10):1101–1115. doi: 10.1161/CIRCRESAHA.116.309402

Figure 2. Nicotine Infusion for 2 Weeks Induces Expansion of CD161a+ Immune Cells in the SHR In Vivo.

Figure 2

Young (3–4 week old) WKY (n=12) and SHR (n=12) were implanted with osmotic pumps infusing either saline (black bars, n=6) or nicotine (15mg/kg/day) (red bars, n=6) for 2 weeks (A). Cells were isolated from the spleens and bone marrow and flow cytometry was performed to assess the presence of CD161a+ immune cells. Nicotine induced proliferation of CD161a+ immune cells in vivo in the bone marrow of the SHR and WKY. The most prominent increase was in splenocytes of SHR with nicotine. Results were compared using two-way ANOVA. Error bars represent the standard error of the mean (SEM) and p-values as indicated.