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. Author manuscript; available in PMC: 2017 Nov 6.
Published in final edited form as: J Mol Biol. 2016 Sep 23;428(22):4503–4519. doi: 10.1016/j.jmb.2016.09.016

Figure 1. Beryllofluoride (BeF3−) promotes formation of Efm LiaR dimers.

Figure 1

Sedimentation equilibrium analytical ultracentrifugation (AUC) profiles for: (a) full length Efm LiaR (dark blue); Efm LiaR with BeF3− (magenta); adaptive mutant Efm LiaRW73C (green); and adaptive mutant Efm LiaRW73C with BeF3− (cyan). (b) AUC profiles for Efm LiaR(RD) (blue); Efm LiaR(RD) with BeF3− (magenta); Efm LiaR(RD)W73C (green); and Efm LiaR(RD)W73C with BeF3− (cyan): (c) AUC profile for the DNA binding domain of Efm LiaR (LiaR(DBD)) alone (magenta). For each protein, the SEQ profiles were globally fitted to a monomer only or monomer-dimer equilibrium model.